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agarose bound galanthus nivalis lectin resin  (Vector Laboratories)


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    Vector Laboratories agarose bound galanthus nivalis lectin resin
    Agarose Bound Galanthus Nivalis Lectin Resin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/AL-1243-5/Agarose+bound+Galanthus+Nivalis+Lectin+(GNL)/pm42047685-239-11-16
    Average 95 stars, based on 141 article reviews
    agarose bound galanthus nivalis lectin resin - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Purification:

    Article Title: Germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaques.
    Article Snippet: .. Purification was carried out using affinity chromatography with either agarose-bound Galanthus nivalis lectin (GNL) (elution with 1M MMP, α-methylmannoside) (Vector Labs, cat #AL-1243-5) or TOYOPEARL AF-Tresyl-650M beads (TOSOH, cat #0014472) conjugated to the PGT145 broadly neutralizing antibody (bnAb) per bead manufacturer’s instructions. .. To ensure further purification, the eluates were subjected to size-exclusion chromatography (SEC) using a Superdex 200 increase 10/300 GL column (GE Healthcare, cat #GE28-9909-44) in PBS.

    Article Title: Early clonal dominance at priming sets the trajectory for broad HIV serum neutralization
    Article Snippet: .. Trimers were purified by affinity chromatography using either agarose-bound Galanthus nivalis lectin (GNL; Vector Labs, Cat# AL-1243-5) or TOYOPEARL AF-Tresyl-650M beads (TOSOH, Cat# 0014472) conjugated to the PGT145 broadly neutralizing antibody (bnAb). .. Eluates were further polished by size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Cat# GE28-9909-44) equilibrated in PBS.

    Affinity Chromatography:

    Article Title: Germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaques.
    Article Snippet: .. Purification was carried out using affinity chromatography with either agarose-bound Galanthus nivalis lectin (GNL) (elution with 1M MMP, α-methylmannoside) (Vector Labs, cat #AL-1243-5) or TOYOPEARL AF-Tresyl-650M beads (TOSOH, cat #0014472) conjugated to the PGT145 broadly neutralizing antibody (bnAb) per bead manufacturer’s instructions. .. To ensure further purification, the eluates were subjected to size-exclusion chromatography (SEC) using a Superdex 200 increase 10/300 GL column (GE Healthcare, cat #GE28-9909-44) in PBS.

    Article Title: Early clonal dominance at priming sets the trajectory for broad HIV serum neutralization
    Article Snippet: .. Trimers were purified by affinity chromatography using either agarose-bound Galanthus nivalis lectin (GNL; Vector Labs, Cat# AL-1243-5) or TOYOPEARL AF-Tresyl-650M beads (TOSOH, Cat# 0014472) conjugated to the PGT145 broadly neutralizing antibody (bnAb). .. Eluates were further polished by size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Cat# GE28-9909-44) equilibrated in PBS.

    other:

    Article Title: Antibody-mediated diversification of primary and secondary humoral immune responses
    Article Snippet: Transient transfections of expression plasmids were performed using the ExpiFectamine 293 transfection kit (A14525; Gibco) as previously described ( ).

    Article Title: Antibody-mediated diversification of primary and secondary humoral immune responses.
    Article Snippet: All recombinant proteins were produced in Expi293F cells (A14527; Gibco), except 426c.WT.SOSIP, which was produced in Expi293F GnTI- cells (A39240; Gibco).

    Membrane:

    Article Title: Germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaques.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM reconstruction and structure of CH42-Apex1.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW0 EMDB: 49870 Cryo-EM reconstruction and structure of CH42-Apex2.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW1 EMDB: 49871 NGS Sequencing Runs This study NCBI SRA: SRX27822716-SRX27822762 Experimental models: Cell lines Expi293F cells Fisher Cat# A14527 HEK293F cells Fisher Cat# R79007 HEK293T cells ATCC Cat# CRL-3216 TZM-b1 cells NIH AIDS Reagents Program N/A Software and algorithms IMGT/V-Quest International ImMunoGeneTics Information System; Marie-Paule Lefranc (marie-paule. lefranc@igh.cnrs.fr), University of Montpellier, France www.imgt.org; RRID: SCR_012780 AbStar Bryan Briney (briney@scripps.edu), The Scripps Research Institute https://github.com/briney/abstar Cellranger 10X Genomics https://support.10xgenomics.com/single- cell-gene-expression/software/downloads/ latest Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ ForteBio Data Analysis software Sartorius https://www.sartorius.com/en PyMOL V2.4.2 PyMOL by Schrödinger https://pymol.org UCSF Chimera Pettersen et al.95 http://plato.cgl.ucsf.edu/chimera/; RRID: SCR_004097 FlowJo v.10 BD Life Sciences https://www.flowjo.com/solutions/flowjo NumPy https://github.com/numpy/numpy v1.20.3 SciPy https://github.com/scipy/scipy v1.6.0 scikit-learn https://github.com/scikit-learn/scikit-learn V0.24.2 Statsmodels https://github.com/statsmodels/ statsmodels v0.12.2 Clustal Omega http://www.clustal.org/omega/ v1.2.2 DiversityAnalyzer https://immunotools.github.io/ immunotools/diversity_analyzer.html N/A Sulfinator https://web.expasy.org/sulfinator/ N/A Iroki https://www.iroki.net/viewer N/A IgDiscover https://gkhlab.gitlab.io/igdiscover22/ V1.0.2 ByosTM (Version 5.5) Protein Metrics Inc. https://www.proteinmetrics.com/products/ byonic/ XCalibur Version v4.2 Thermo Fisher N/A Orbitrap Fusion Tune application v3.1 Thermo Fisher N/A UCSF ChimeraX Meng et al.96 https://www.cgl.ucsf.edu/chimerax/; RRID:SCR_015872 Relion 4.0 Kimanius et al.97 RRID:SCR_016274 EPU Multigrid Thermo Fisher N/A CryoSPARC Punjani et al.98 https://cryosparc.com UCSF Chimera Pettersen et al.95 https://www.cgl.ucsf.edu/chimera/ Phenix Liebschner et al.99 https://phenix-online.org PDBePISA Krissinel and Henrick100 http://www.ebi.ac.uk/pdbe/pisa/ (Continued on next page) Immunity 59, 1140–1160.e1–e11, April 14, 2026 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Coot Emsley and Cowtan101 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ MolProbity Chen et al.102 http://molprobity.biochem.duke.edu EMRinger Barad et al.103 https://github.com/fraser-lab/EMRinger ABodyBuilder2 Abanades et al.104 https://opig.stats.ox.ac.uk/webapps/ sabdab-sabpred/sabpred/abodybuilder2/ Other 0.2 um membrane filters Fisher Scientific Cat# 564-0020 SteriflipTM Vacuum Filter Units MilliporeSigma Cat# SCGP00525 Superdex 200 Increase10/300 GL column GE Healthcare Cat# GE28-9909-44 Superose 6 Increase 10/300 GL GE Healthcare Cat# GE29-0915-96 Protein A Sepharose Cytiva Cat# 17127903 Protein G Sepharose Cytiva Cat# 17061805 TOYOPEARL AF-Tresyl-650M TOSOH Cat# 0014472 Galanthus nivalis lectin (snow drop), agarose bound Vector Labs Cat# AL-1243-5 ProA Sensor Sartorius Cat# 18-5012 SA Sensor Sartorius Cat# 18-5020 RNeasy Plus Mini Kit Qiagen Cat# 74134 Dnase 1 Qiagen Cat# 79254 Streptavidin-AF488 Thermo Fisher Cat# S32354 Streptavidin-AF647 Thermo Fisher Cat# S21374 Streptavidin-BV421 BD Biosciences Cat# 563259 FVS510 Live/Dead stain Thermo Fisher Scientific Cat# L34966 Chromium Next GEM Single Cell 5’ Kit v2 10X Genomics Cat# 1000253 Chromium Next GEM Chip K Single Cell Kit 10X Genomics Cat# 1000286 NexttSeq 1000/2000 P1 Reagent Cartridge Illumina Cat# 20072323 E-Gel 96 2% with SYVR Safe Fisher Scientificd Cat# G720802 Econo-pak column Biorad Cat# 7321010 100kDA Ultra centrifugal filter unit Millipore Cat# UFC910024 50kDA Ultra centrifugal filter unit Millipore Cat# UFC905024 10kDA Ultra centrifugal filter unit Millipore Cat# UFC901024 Pierce Fab Preparation Kit Fisher Scientific Cat# 44985 cryovials Globe Scientific Cat# 3010 Sterile vacutainers DB vacutainer Cat# 364606 cryovial Sarstedt Cat# 72.694.396 ViaStain AOPI solution Revvity Cat# CS2-0106-25ml Cell strainer Falcon Cat# 352360 5 ml round bottom tube Corning Cat# 352058 C18 ZipTip Merck Milipore Cat# ZTC18S008 Oasis HLB 96-well μElution Plate Waters Cat# 186001828BA Vivaspin 500, 3 kDa MWCO, Polyethersulfone Sigma-Aldrich Cat# GE28-9322-18 Orbitrap Fusion mass spectrometer Thermo Fisher Scientific N/A Easy nLC 1200 Thermo Fisher Scientific N/A EasySpray PepMap RSLC C18 column (75 μm x 75 cm) Thermo Fisher Scientific Cat# ES805 AcclaimTM PepMapTM 100 C18 HPLC Trap column Thermo Fisher Scientific Cat# 164946 Electron microscopy copper mesh grids Electron Microscopy Sciences Cat# EMS400-Cu Whatman #1 filter paper Cytiva Cat# 1001-320 (Continued on next page) e4 Immunity 59, 1140–1160.e1–e11, April 14, 2026 .. Expi293F cells (Gibco, Cat# A14527) were acquired from Gibco and cultured in Expi293 Expression Medium (Gibco, Cat# A1435101) at 37◦C with 8% CO2 in a 125 rpm shaker.

    Staining:

    Article Title: Germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaques.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM reconstruction and structure of CH42-Apex1.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW0 EMDB: 49870 Cryo-EM reconstruction and structure of CH42-Apex2.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW1 EMDB: 49871 NGS Sequencing Runs This study NCBI SRA: SRX27822716-SRX27822762 Experimental models: Cell lines Expi293F cells Fisher Cat# A14527 HEK293F cells Fisher Cat# R79007 HEK293T cells ATCC Cat# CRL-3216 TZM-b1 cells NIH AIDS Reagents Program N/A Software and algorithms IMGT/V-Quest International ImMunoGeneTics Information System; Marie-Paule Lefranc (marie-paule. lefranc@igh.cnrs.fr), University of Montpellier, France www.imgt.org; RRID: SCR_012780 AbStar Bryan Briney (briney@scripps.edu), The Scripps Research Institute https://github.com/briney/abstar Cellranger 10X Genomics https://support.10xgenomics.com/single- cell-gene-expression/software/downloads/ latest Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ ForteBio Data Analysis software Sartorius https://www.sartorius.com/en PyMOL V2.4.2 PyMOL by Schrödinger https://pymol.org UCSF Chimera Pettersen et al.95 http://plato.cgl.ucsf.edu/chimera/; RRID: SCR_004097 FlowJo v.10 BD Life Sciences https://www.flowjo.com/solutions/flowjo NumPy https://github.com/numpy/numpy v1.20.3 SciPy https://github.com/scipy/scipy v1.6.0 scikit-learn https://github.com/scikit-learn/scikit-learn V0.24.2 Statsmodels https://github.com/statsmodels/ statsmodels v0.12.2 Clustal Omega http://www.clustal.org/omega/ v1.2.2 DiversityAnalyzer https://immunotools.github.io/ immunotools/diversity_analyzer.html N/A Sulfinator https://web.expasy.org/sulfinator/ N/A Iroki https://www.iroki.net/viewer N/A IgDiscover https://gkhlab.gitlab.io/igdiscover22/ V1.0.2 ByosTM (Version 5.5) Protein Metrics Inc. https://www.proteinmetrics.com/products/ byonic/ XCalibur Version v4.2 Thermo Fisher N/A Orbitrap Fusion Tune application v3.1 Thermo Fisher N/A UCSF ChimeraX Meng et al.96 https://www.cgl.ucsf.edu/chimerax/; RRID:SCR_015872 Relion 4.0 Kimanius et al.97 RRID:SCR_016274 EPU Multigrid Thermo Fisher N/A CryoSPARC Punjani et al.98 https://cryosparc.com UCSF Chimera Pettersen et al.95 https://www.cgl.ucsf.edu/chimera/ Phenix Liebschner et al.99 https://phenix-online.org PDBePISA Krissinel and Henrick100 http://www.ebi.ac.uk/pdbe/pisa/ (Continued on next page) Immunity 59, 1140–1160.e1–e11, April 14, 2026 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Coot Emsley and Cowtan101 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ MolProbity Chen et al.102 http://molprobity.biochem.duke.edu EMRinger Barad et al.103 https://github.com/fraser-lab/EMRinger ABodyBuilder2 Abanades et al.104 https://opig.stats.ox.ac.uk/webapps/ sabdab-sabpred/sabpred/abodybuilder2/ Other 0.2 um membrane filters Fisher Scientific Cat# 564-0020 SteriflipTM Vacuum Filter Units MilliporeSigma Cat# SCGP00525 Superdex 200 Increase10/300 GL column GE Healthcare Cat# GE28-9909-44 Superose 6 Increase 10/300 GL GE Healthcare Cat# GE29-0915-96 Protein A Sepharose Cytiva Cat# 17127903 Protein G Sepharose Cytiva Cat# 17061805 TOYOPEARL AF-Tresyl-650M TOSOH Cat# 0014472 Galanthus nivalis lectin (snow drop), agarose bound Vector Labs Cat# AL-1243-5 ProA Sensor Sartorius Cat# 18-5012 SA Sensor Sartorius Cat# 18-5020 RNeasy Plus Mini Kit Qiagen Cat# 74134 Dnase 1 Qiagen Cat# 79254 Streptavidin-AF488 Thermo Fisher Cat# S32354 Streptavidin-AF647 Thermo Fisher Cat# S21374 Streptavidin-BV421 BD Biosciences Cat# 563259 FVS510 Live/Dead stain Thermo Fisher Scientific Cat# L34966 Chromium Next GEM Single Cell 5’ Kit v2 10X Genomics Cat# 1000253 Chromium Next GEM Chip K Single Cell Kit 10X Genomics Cat# 1000286 NexttSeq 1000/2000 P1 Reagent Cartridge Illumina Cat# 20072323 E-Gel 96 2% with SYVR Safe Fisher Scientificd Cat# G720802 Econo-pak column Biorad Cat# 7321010 100kDA Ultra centrifugal filter unit Millipore Cat# UFC910024 50kDA Ultra centrifugal filter unit Millipore Cat# UFC905024 10kDA Ultra centrifugal filter unit Millipore Cat# UFC901024 Pierce Fab Preparation Kit Fisher Scientific Cat# 44985 cryovials Globe Scientific Cat# 3010 Sterile vacutainers DB vacutainer Cat# 364606 cryovial Sarstedt Cat# 72.694.396 ViaStain AOPI solution Revvity Cat# CS2-0106-25ml Cell strainer Falcon Cat# 352360 5 ml round bottom tube Corning Cat# 352058 C18 ZipTip Merck Milipore Cat# ZTC18S008 Oasis HLB 96-well μElution Plate Waters Cat# 186001828BA Vivaspin 500, 3 kDa MWCO, Polyethersulfone Sigma-Aldrich Cat# GE28-9322-18 Orbitrap Fusion mass spectrometer Thermo Fisher Scientific N/A Easy nLC 1200 Thermo Fisher Scientific N/A EasySpray PepMap RSLC C18 column (75 μm x 75 cm) Thermo Fisher Scientific Cat# ES805 AcclaimTM PepMapTM 100 C18 HPLC Trap column Thermo Fisher Scientific Cat# 164946 Electron microscopy copper mesh grids Electron Microscopy Sciences Cat# EMS400-Cu Whatman #1 filter paper Cytiva Cat# 1001-320 (Continued on next page) e4 Immunity 59, 1140–1160.e1–e11, April 14, 2026 .. Expi293F cells (Gibco, Cat# A14527) were acquired from Gibco and cultured in Expi293 Expression Medium (Gibco, Cat# A1435101) at 37◦C with 8% CO2 in a 125 rpm shaker.

    Single Cell:

    Article Title: Germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaques.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM reconstruction and structure of CH42-Apex1.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW0 EMDB: 49870 Cryo-EM reconstruction and structure of CH42-Apex2.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW1 EMDB: 49871 NGS Sequencing Runs This study NCBI SRA: SRX27822716-SRX27822762 Experimental models: Cell lines Expi293F cells Fisher Cat# A14527 HEK293F cells Fisher Cat# R79007 HEK293T cells ATCC Cat# CRL-3216 TZM-b1 cells NIH AIDS Reagents Program N/A Software and algorithms IMGT/V-Quest International ImMunoGeneTics Information System; Marie-Paule Lefranc (marie-paule. lefranc@igh.cnrs.fr), University of Montpellier, France www.imgt.org; RRID: SCR_012780 AbStar Bryan Briney (briney@scripps.edu), The Scripps Research Institute https://github.com/briney/abstar Cellranger 10X Genomics https://support.10xgenomics.com/single- cell-gene-expression/software/downloads/ latest Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ ForteBio Data Analysis software Sartorius https://www.sartorius.com/en PyMOL V2.4.2 PyMOL by Schrödinger https://pymol.org UCSF Chimera Pettersen et al.95 http://plato.cgl.ucsf.edu/chimera/; RRID: SCR_004097 FlowJo v.10 BD Life Sciences https://www.flowjo.com/solutions/flowjo NumPy https://github.com/numpy/numpy v1.20.3 SciPy https://github.com/scipy/scipy v1.6.0 scikit-learn https://github.com/scikit-learn/scikit-learn V0.24.2 Statsmodels https://github.com/statsmodels/ statsmodels v0.12.2 Clustal Omega http://www.clustal.org/omega/ v1.2.2 DiversityAnalyzer https://immunotools.github.io/ immunotools/diversity_analyzer.html N/A Sulfinator https://web.expasy.org/sulfinator/ N/A Iroki https://www.iroki.net/viewer N/A IgDiscover https://gkhlab.gitlab.io/igdiscover22/ V1.0.2 ByosTM (Version 5.5) Protein Metrics Inc. https://www.proteinmetrics.com/products/ byonic/ XCalibur Version v4.2 Thermo Fisher N/A Orbitrap Fusion Tune application v3.1 Thermo Fisher N/A UCSF ChimeraX Meng et al.96 https://www.cgl.ucsf.edu/chimerax/; RRID:SCR_015872 Relion 4.0 Kimanius et al.97 RRID:SCR_016274 EPU Multigrid Thermo Fisher N/A CryoSPARC Punjani et al.98 https://cryosparc.com UCSF Chimera Pettersen et al.95 https://www.cgl.ucsf.edu/chimera/ Phenix Liebschner et al.99 https://phenix-online.org PDBePISA Krissinel and Henrick100 http://www.ebi.ac.uk/pdbe/pisa/ (Continued on next page) Immunity 59, 1140–1160.e1–e11, April 14, 2026 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Coot Emsley and Cowtan101 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ MolProbity Chen et al.102 http://molprobity.biochem.duke.edu EMRinger Barad et al.103 https://github.com/fraser-lab/EMRinger ABodyBuilder2 Abanades et al.104 https://opig.stats.ox.ac.uk/webapps/ sabdab-sabpred/sabpred/abodybuilder2/ Other 0.2 um membrane filters Fisher Scientific Cat# 564-0020 SteriflipTM Vacuum Filter Units MilliporeSigma Cat# SCGP00525 Superdex 200 Increase10/300 GL column GE Healthcare Cat# GE28-9909-44 Superose 6 Increase 10/300 GL GE Healthcare Cat# GE29-0915-96 Protein A Sepharose Cytiva Cat# 17127903 Protein G Sepharose Cytiva Cat# 17061805 TOYOPEARL AF-Tresyl-650M TOSOH Cat# 0014472 Galanthus nivalis lectin (snow drop), agarose bound Vector Labs Cat# AL-1243-5 ProA Sensor Sartorius Cat# 18-5012 SA Sensor Sartorius Cat# 18-5020 RNeasy Plus Mini Kit Qiagen Cat# 74134 Dnase 1 Qiagen Cat# 79254 Streptavidin-AF488 Thermo Fisher Cat# S32354 Streptavidin-AF647 Thermo Fisher Cat# S21374 Streptavidin-BV421 BD Biosciences Cat# 563259 FVS510 Live/Dead stain Thermo Fisher Scientific Cat# L34966 Chromium Next GEM Single Cell 5’ Kit v2 10X Genomics Cat# 1000253 Chromium Next GEM Chip K Single Cell Kit 10X Genomics Cat# 1000286 NexttSeq 1000/2000 P1 Reagent Cartridge Illumina Cat# 20072323 E-Gel 96 2% with SYVR Safe Fisher Scientificd Cat# G720802 Econo-pak column Biorad Cat# 7321010 100kDA Ultra centrifugal filter unit Millipore Cat# UFC910024 50kDA Ultra centrifugal filter unit Millipore Cat# UFC905024 10kDA Ultra centrifugal filter unit Millipore Cat# UFC901024 Pierce Fab Preparation Kit Fisher Scientific Cat# 44985 cryovials Globe Scientific Cat# 3010 Sterile vacutainers DB vacutainer Cat# 364606 cryovial Sarstedt Cat# 72.694.396 ViaStain AOPI solution Revvity Cat# CS2-0106-25ml Cell strainer Falcon Cat# 352360 5 ml round bottom tube Corning Cat# 352058 C18 ZipTip Merck Milipore Cat# ZTC18S008 Oasis HLB 96-well μElution Plate Waters Cat# 186001828BA Vivaspin 500, 3 kDa MWCO, Polyethersulfone Sigma-Aldrich Cat# GE28-9322-18 Orbitrap Fusion mass spectrometer Thermo Fisher Scientific N/A Easy nLC 1200 Thermo Fisher Scientific N/A EasySpray PepMap RSLC C18 column (75 μm x 75 cm) Thermo Fisher Scientific Cat# ES805 AcclaimTM PepMapTM 100 C18 HPLC Trap column Thermo Fisher Scientific Cat# 164946 Electron microscopy copper mesh grids Electron Microscopy Sciences Cat# EMS400-Cu Whatman #1 filter paper Cytiva Cat# 1001-320 (Continued on next page) e4 Immunity 59, 1140–1160.e1–e11, April 14, 2026 .. Expi293F cells (Gibco, Cat# A14527) were acquired from Gibco and cultured in Expi293 Expression Medium (Gibco, Cat# A1435101) at 37◦C with 8% CO2 in a 125 rpm shaker.

    Sterility:

    Article Title: Germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaques.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM reconstruction and structure of CH42-Apex1.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW0 EMDB: 49870 Cryo-EM reconstruction and structure of CH42-Apex2.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW1 EMDB: 49871 NGS Sequencing Runs This study NCBI SRA: SRX27822716-SRX27822762 Experimental models: Cell lines Expi293F cells Fisher Cat# A14527 HEK293F cells Fisher Cat# R79007 HEK293T cells ATCC Cat# CRL-3216 TZM-b1 cells NIH AIDS Reagents Program N/A Software and algorithms IMGT/V-Quest International ImMunoGeneTics Information System; Marie-Paule Lefranc (marie-paule. lefranc@igh.cnrs.fr), University of Montpellier, France www.imgt.org; RRID: SCR_012780 AbStar Bryan Briney (briney@scripps.edu), The Scripps Research Institute https://github.com/briney/abstar Cellranger 10X Genomics https://support.10xgenomics.com/single- cell-gene-expression/software/downloads/ latest Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ ForteBio Data Analysis software Sartorius https://www.sartorius.com/en PyMOL V2.4.2 PyMOL by Schrödinger https://pymol.org UCSF Chimera Pettersen et al.95 http://plato.cgl.ucsf.edu/chimera/; RRID: SCR_004097 FlowJo v.10 BD Life Sciences https://www.flowjo.com/solutions/flowjo NumPy https://github.com/numpy/numpy v1.20.3 SciPy https://github.com/scipy/scipy v1.6.0 scikit-learn https://github.com/scikit-learn/scikit-learn V0.24.2 Statsmodels https://github.com/statsmodels/ statsmodels v0.12.2 Clustal Omega http://www.clustal.org/omega/ v1.2.2 DiversityAnalyzer https://immunotools.github.io/ immunotools/diversity_analyzer.html N/A Sulfinator https://web.expasy.org/sulfinator/ N/A Iroki https://www.iroki.net/viewer N/A IgDiscover https://gkhlab.gitlab.io/igdiscover22/ V1.0.2 ByosTM (Version 5.5) Protein Metrics Inc. https://www.proteinmetrics.com/products/ byonic/ XCalibur Version v4.2 Thermo Fisher N/A Orbitrap Fusion Tune application v3.1 Thermo Fisher N/A UCSF ChimeraX Meng et al.96 https://www.cgl.ucsf.edu/chimerax/; RRID:SCR_015872 Relion 4.0 Kimanius et al.97 RRID:SCR_016274 EPU Multigrid Thermo Fisher N/A CryoSPARC Punjani et al.98 https://cryosparc.com UCSF Chimera Pettersen et al.95 https://www.cgl.ucsf.edu/chimera/ Phenix Liebschner et al.99 https://phenix-online.org PDBePISA Krissinel and Henrick100 http://www.ebi.ac.uk/pdbe/pisa/ (Continued on next page) Immunity 59, 1140–1160.e1–e11, April 14, 2026 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Coot Emsley and Cowtan101 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ MolProbity Chen et al.102 http://molprobity.biochem.duke.edu EMRinger Barad et al.103 https://github.com/fraser-lab/EMRinger ABodyBuilder2 Abanades et al.104 https://opig.stats.ox.ac.uk/webapps/ sabdab-sabpred/sabpred/abodybuilder2/ Other 0.2 um membrane filters Fisher Scientific Cat# 564-0020 SteriflipTM Vacuum Filter Units MilliporeSigma Cat# SCGP00525 Superdex 200 Increase10/300 GL column GE Healthcare Cat# GE28-9909-44 Superose 6 Increase 10/300 GL GE Healthcare Cat# GE29-0915-96 Protein A Sepharose Cytiva Cat# 17127903 Protein G Sepharose Cytiva Cat# 17061805 TOYOPEARL AF-Tresyl-650M TOSOH Cat# 0014472 Galanthus nivalis lectin (snow drop), agarose bound Vector Labs Cat# AL-1243-5 ProA Sensor Sartorius Cat# 18-5012 SA Sensor Sartorius Cat# 18-5020 RNeasy Plus Mini Kit Qiagen Cat# 74134 Dnase 1 Qiagen Cat# 79254 Streptavidin-AF488 Thermo Fisher Cat# S32354 Streptavidin-AF647 Thermo Fisher Cat# S21374 Streptavidin-BV421 BD Biosciences Cat# 563259 FVS510 Live/Dead stain Thermo Fisher Scientific Cat# L34966 Chromium Next GEM Single Cell 5’ Kit v2 10X Genomics Cat# 1000253 Chromium Next GEM Chip K Single Cell Kit 10X Genomics Cat# 1000286 NexttSeq 1000/2000 P1 Reagent Cartridge Illumina Cat# 20072323 E-Gel 96 2% with SYVR Safe Fisher Scientificd Cat# G720802 Econo-pak column Biorad Cat# 7321010 100kDA Ultra centrifugal filter unit Millipore Cat# UFC910024 50kDA Ultra centrifugal filter unit Millipore Cat# UFC905024 10kDA Ultra centrifugal filter unit Millipore Cat# UFC901024 Pierce Fab Preparation Kit Fisher Scientific Cat# 44985 cryovials Globe Scientific Cat# 3010 Sterile vacutainers DB vacutainer Cat# 364606 cryovial Sarstedt Cat# 72.694.396 ViaStain AOPI solution Revvity Cat# CS2-0106-25ml Cell strainer Falcon Cat# 352360 5 ml round bottom tube Corning Cat# 352058 C18 ZipTip Merck Milipore Cat# ZTC18S008 Oasis HLB 96-well μElution Plate Waters Cat# 186001828BA Vivaspin 500, 3 kDa MWCO, Polyethersulfone Sigma-Aldrich Cat# GE28-9322-18 Orbitrap Fusion mass spectrometer Thermo Fisher Scientific N/A Easy nLC 1200 Thermo Fisher Scientific N/A EasySpray PepMap RSLC C18 column (75 μm x 75 cm) Thermo Fisher Scientific Cat# ES805 AcclaimTM PepMapTM 100 C18 HPLC Trap column Thermo Fisher Scientific Cat# 164946 Electron microscopy copper mesh grids Electron Microscopy Sciences Cat# EMS400-Cu Whatman #1 filter paper Cytiva Cat# 1001-320 (Continued on next page) e4 Immunity 59, 1140–1160.e1–e11, April 14, 2026 .. Expi293F cells (Gibco, Cat# A14527) were acquired from Gibco and cultured in Expi293 Expression Medium (Gibco, Cat# A1435101) at 37◦C with 8% CO2 in a 125 rpm shaker.

    Mass Spectrometry:

    Article Title: Germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaques.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM reconstruction and structure of CH42-Apex1.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW0 EMDB: 49870 Cryo-EM reconstruction and structure of CH42-Apex2.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW1 EMDB: 49871 NGS Sequencing Runs This study NCBI SRA: SRX27822716-SRX27822762 Experimental models: Cell lines Expi293F cells Fisher Cat# A14527 HEK293F cells Fisher Cat# R79007 HEK293T cells ATCC Cat# CRL-3216 TZM-b1 cells NIH AIDS Reagents Program N/A Software and algorithms IMGT/V-Quest International ImMunoGeneTics Information System; Marie-Paule Lefranc (marie-paule. lefranc@igh.cnrs.fr), University of Montpellier, France www.imgt.org; RRID: SCR_012780 AbStar Bryan Briney (briney@scripps.edu), The Scripps Research Institute https://github.com/briney/abstar Cellranger 10X Genomics https://support.10xgenomics.com/single- cell-gene-expression/software/downloads/ latest Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ ForteBio Data Analysis software Sartorius https://www.sartorius.com/en PyMOL V2.4.2 PyMOL by Schrödinger https://pymol.org UCSF Chimera Pettersen et al.95 http://plato.cgl.ucsf.edu/chimera/; RRID: SCR_004097 FlowJo v.10 BD Life Sciences https://www.flowjo.com/solutions/flowjo NumPy https://github.com/numpy/numpy v1.20.3 SciPy https://github.com/scipy/scipy v1.6.0 scikit-learn https://github.com/scikit-learn/scikit-learn V0.24.2 Statsmodels https://github.com/statsmodels/ statsmodels v0.12.2 Clustal Omega http://www.clustal.org/omega/ v1.2.2 DiversityAnalyzer https://immunotools.github.io/ immunotools/diversity_analyzer.html N/A Sulfinator https://web.expasy.org/sulfinator/ N/A Iroki https://www.iroki.net/viewer N/A IgDiscover https://gkhlab.gitlab.io/igdiscover22/ V1.0.2 ByosTM (Version 5.5) Protein Metrics Inc. https://www.proteinmetrics.com/products/ byonic/ XCalibur Version v4.2 Thermo Fisher N/A Orbitrap Fusion Tune application v3.1 Thermo Fisher N/A UCSF ChimeraX Meng et al.96 https://www.cgl.ucsf.edu/chimerax/; RRID:SCR_015872 Relion 4.0 Kimanius et al.97 RRID:SCR_016274 EPU Multigrid Thermo Fisher N/A CryoSPARC Punjani et al.98 https://cryosparc.com UCSF Chimera Pettersen et al.95 https://www.cgl.ucsf.edu/chimera/ Phenix Liebschner et al.99 https://phenix-online.org PDBePISA Krissinel and Henrick100 http://www.ebi.ac.uk/pdbe/pisa/ (Continued on next page) Immunity 59, 1140–1160.e1–e11, April 14, 2026 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Coot Emsley and Cowtan101 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ MolProbity Chen et al.102 http://molprobity.biochem.duke.edu EMRinger Barad et al.103 https://github.com/fraser-lab/EMRinger ABodyBuilder2 Abanades et al.104 https://opig.stats.ox.ac.uk/webapps/ sabdab-sabpred/sabpred/abodybuilder2/ Other 0.2 um membrane filters Fisher Scientific Cat# 564-0020 SteriflipTM Vacuum Filter Units MilliporeSigma Cat# SCGP00525 Superdex 200 Increase10/300 GL column GE Healthcare Cat# GE28-9909-44 Superose 6 Increase 10/300 GL GE Healthcare Cat# GE29-0915-96 Protein A Sepharose Cytiva Cat# 17127903 Protein G Sepharose Cytiva Cat# 17061805 TOYOPEARL AF-Tresyl-650M TOSOH Cat# 0014472 Galanthus nivalis lectin (snow drop), agarose bound Vector Labs Cat# AL-1243-5 ProA Sensor Sartorius Cat# 18-5012 SA Sensor Sartorius Cat# 18-5020 RNeasy Plus Mini Kit Qiagen Cat# 74134 Dnase 1 Qiagen Cat# 79254 Streptavidin-AF488 Thermo Fisher Cat# S32354 Streptavidin-AF647 Thermo Fisher Cat# S21374 Streptavidin-BV421 BD Biosciences Cat# 563259 FVS510 Live/Dead stain Thermo Fisher Scientific Cat# L34966 Chromium Next GEM Single Cell 5’ Kit v2 10X Genomics Cat# 1000253 Chromium Next GEM Chip K Single Cell Kit 10X Genomics Cat# 1000286 NexttSeq 1000/2000 P1 Reagent Cartridge Illumina Cat# 20072323 E-Gel 96 2% with SYVR Safe Fisher Scientificd Cat# G720802 Econo-pak column Biorad Cat# 7321010 100kDA Ultra centrifugal filter unit Millipore Cat# UFC910024 50kDA Ultra centrifugal filter unit Millipore Cat# UFC905024 10kDA Ultra centrifugal filter unit Millipore Cat# UFC901024 Pierce Fab Preparation Kit Fisher Scientific Cat# 44985 cryovials Globe Scientific Cat# 3010 Sterile vacutainers DB vacutainer Cat# 364606 cryovial Sarstedt Cat# 72.694.396 ViaStain AOPI solution Revvity Cat# CS2-0106-25ml Cell strainer Falcon Cat# 352360 5 ml round bottom tube Corning Cat# 352058 C18 ZipTip Merck Milipore Cat# ZTC18S008 Oasis HLB 96-well μElution Plate Waters Cat# 186001828BA Vivaspin 500, 3 kDa MWCO, Polyethersulfone Sigma-Aldrich Cat# GE28-9322-18 Orbitrap Fusion mass spectrometer Thermo Fisher Scientific N/A Easy nLC 1200 Thermo Fisher Scientific N/A EasySpray PepMap RSLC C18 column (75 μm x 75 cm) Thermo Fisher Scientific Cat# ES805 AcclaimTM PepMapTM 100 C18 HPLC Trap column Thermo Fisher Scientific Cat# 164946 Electron microscopy copper mesh grids Electron Microscopy Sciences Cat# EMS400-Cu Whatman #1 filter paper Cytiva Cat# 1001-320 (Continued on next page) e4 Immunity 59, 1140–1160.e1–e11, April 14, 2026 .. Expi293F cells (Gibco, Cat# A14527) were acquired from Gibco and cultured in Expi293 Expression Medium (Gibco, Cat# A1435101) at 37◦C with 8% CO2 in a 125 rpm shaker.

    High Performance Liquid Chromatography:

    Article Title: Germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaques.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM reconstruction and structure of CH42-Apex1.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW0 EMDB: 49870 Cryo-EM reconstruction and structure of CH42-Apex2.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW1 EMDB: 49871 NGS Sequencing Runs This study NCBI SRA: SRX27822716-SRX27822762 Experimental models: Cell lines Expi293F cells Fisher Cat# A14527 HEK293F cells Fisher Cat# R79007 HEK293T cells ATCC Cat# CRL-3216 TZM-b1 cells NIH AIDS Reagents Program N/A Software and algorithms IMGT/V-Quest International ImMunoGeneTics Information System; Marie-Paule Lefranc (marie-paule. lefranc@igh.cnrs.fr), University of Montpellier, France www.imgt.org; RRID: SCR_012780 AbStar Bryan Briney (briney@scripps.edu), The Scripps Research Institute https://github.com/briney/abstar Cellranger 10X Genomics https://support.10xgenomics.com/single- cell-gene-expression/software/downloads/ latest Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ ForteBio Data Analysis software Sartorius https://www.sartorius.com/en PyMOL V2.4.2 PyMOL by Schrödinger https://pymol.org UCSF Chimera Pettersen et al.95 http://plato.cgl.ucsf.edu/chimera/; RRID: SCR_004097 FlowJo v.10 BD Life Sciences https://www.flowjo.com/solutions/flowjo NumPy https://github.com/numpy/numpy v1.20.3 SciPy https://github.com/scipy/scipy v1.6.0 scikit-learn https://github.com/scikit-learn/scikit-learn V0.24.2 Statsmodels https://github.com/statsmodels/ statsmodels v0.12.2 Clustal Omega http://www.clustal.org/omega/ v1.2.2 DiversityAnalyzer https://immunotools.github.io/ immunotools/diversity_analyzer.html N/A Sulfinator https://web.expasy.org/sulfinator/ N/A Iroki https://www.iroki.net/viewer N/A IgDiscover https://gkhlab.gitlab.io/igdiscover22/ V1.0.2 ByosTM (Version 5.5) Protein Metrics Inc. https://www.proteinmetrics.com/products/ byonic/ XCalibur Version v4.2 Thermo Fisher N/A Orbitrap Fusion Tune application v3.1 Thermo Fisher N/A UCSF ChimeraX Meng et al.96 https://www.cgl.ucsf.edu/chimerax/; RRID:SCR_015872 Relion 4.0 Kimanius et al.97 RRID:SCR_016274 EPU Multigrid Thermo Fisher N/A CryoSPARC Punjani et al.98 https://cryosparc.com UCSF Chimera Pettersen et al.95 https://www.cgl.ucsf.edu/chimera/ Phenix Liebschner et al.99 https://phenix-online.org PDBePISA Krissinel and Henrick100 http://www.ebi.ac.uk/pdbe/pisa/ (Continued on next page) Immunity 59, 1140–1160.e1–e11, April 14, 2026 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Coot Emsley and Cowtan101 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ MolProbity Chen et al.102 http://molprobity.biochem.duke.edu EMRinger Barad et al.103 https://github.com/fraser-lab/EMRinger ABodyBuilder2 Abanades et al.104 https://opig.stats.ox.ac.uk/webapps/ sabdab-sabpred/sabpred/abodybuilder2/ Other 0.2 um membrane filters Fisher Scientific Cat# 564-0020 SteriflipTM Vacuum Filter Units MilliporeSigma Cat# SCGP00525 Superdex 200 Increase10/300 GL column GE Healthcare Cat# GE28-9909-44 Superose 6 Increase 10/300 GL GE Healthcare Cat# GE29-0915-96 Protein A Sepharose Cytiva Cat# 17127903 Protein G Sepharose Cytiva Cat# 17061805 TOYOPEARL AF-Tresyl-650M TOSOH Cat# 0014472 Galanthus nivalis lectin (snow drop), agarose bound Vector Labs Cat# AL-1243-5 ProA Sensor Sartorius Cat# 18-5012 SA Sensor Sartorius Cat# 18-5020 RNeasy Plus Mini Kit Qiagen Cat# 74134 Dnase 1 Qiagen Cat# 79254 Streptavidin-AF488 Thermo Fisher Cat# S32354 Streptavidin-AF647 Thermo Fisher Cat# S21374 Streptavidin-BV421 BD Biosciences Cat# 563259 FVS510 Live/Dead stain Thermo Fisher Scientific Cat# L34966 Chromium Next GEM Single Cell 5’ Kit v2 10X Genomics Cat# 1000253 Chromium Next GEM Chip K Single Cell Kit 10X Genomics Cat# 1000286 NexttSeq 1000/2000 P1 Reagent Cartridge Illumina Cat# 20072323 E-Gel 96 2% with SYVR Safe Fisher Scientificd Cat# G720802 Econo-pak column Biorad Cat# 7321010 100kDA Ultra centrifugal filter unit Millipore Cat# UFC910024 50kDA Ultra centrifugal filter unit Millipore Cat# UFC905024 10kDA Ultra centrifugal filter unit Millipore Cat# UFC901024 Pierce Fab Preparation Kit Fisher Scientific Cat# 44985 cryovials Globe Scientific Cat# 3010 Sterile vacutainers DB vacutainer Cat# 364606 cryovial Sarstedt Cat# 72.694.396 ViaStain AOPI solution Revvity Cat# CS2-0106-25ml Cell strainer Falcon Cat# 352360 5 ml round bottom tube Corning Cat# 352058 C18 ZipTip Merck Milipore Cat# ZTC18S008 Oasis HLB 96-well μElution Plate Waters Cat# 186001828BA Vivaspin 500, 3 kDa MWCO, Polyethersulfone Sigma-Aldrich Cat# GE28-9322-18 Orbitrap Fusion mass spectrometer Thermo Fisher Scientific N/A Easy nLC 1200 Thermo Fisher Scientific N/A EasySpray PepMap RSLC C18 column (75 μm x 75 cm) Thermo Fisher Scientific Cat# ES805 AcclaimTM PepMapTM 100 C18 HPLC Trap column Thermo Fisher Scientific Cat# 164946 Electron microscopy copper mesh grids Electron Microscopy Sciences Cat# EMS400-Cu Whatman #1 filter paper Cytiva Cat# 1001-320 (Continued on next page) e4 Immunity 59, 1140–1160.e1–e11, April 14, 2026 .. Expi293F cells (Gibco, Cat# A14527) were acquired from Gibco and cultured in Expi293 Expression Medium (Gibco, Cat# A1435101) at 37◦C with 8% CO2 in a 125 rpm shaker.

    Electron Microscopy:

    Article Title: Germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaques.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM reconstruction and structure of CH42-Apex1.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW0 EMDB: 49870 Cryo-EM reconstruction and structure of CH42-Apex2.01 Fab in complex with Q23APEX-GT2 This study PDB: 9NW1 EMDB: 49871 NGS Sequencing Runs This study NCBI SRA: SRX27822716-SRX27822762 Experimental models: Cell lines Expi293F cells Fisher Cat# A14527 HEK293F cells Fisher Cat# R79007 HEK293T cells ATCC Cat# CRL-3216 TZM-b1 cells NIH AIDS Reagents Program N/A Software and algorithms IMGT/V-Quest International ImMunoGeneTics Information System; Marie-Paule Lefranc (marie-paule. lefranc@igh.cnrs.fr), University of Montpellier, France www.imgt.org; RRID: SCR_012780 AbStar Bryan Briney (briney@scripps.edu), The Scripps Research Institute https://github.com/briney/abstar Cellranger 10X Genomics https://support.10xgenomics.com/single- cell-gene-expression/software/downloads/ latest Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ ForteBio Data Analysis software Sartorius https://www.sartorius.com/en PyMOL V2.4.2 PyMOL by Schrödinger https://pymol.org UCSF Chimera Pettersen et al.95 http://plato.cgl.ucsf.edu/chimera/; RRID: SCR_004097 FlowJo v.10 BD Life Sciences https://www.flowjo.com/solutions/flowjo NumPy https://github.com/numpy/numpy v1.20.3 SciPy https://github.com/scipy/scipy v1.6.0 scikit-learn https://github.com/scikit-learn/scikit-learn V0.24.2 Statsmodels https://github.com/statsmodels/ statsmodels v0.12.2 Clustal Omega http://www.clustal.org/omega/ v1.2.2 DiversityAnalyzer https://immunotools.github.io/ immunotools/diversity_analyzer.html N/A Sulfinator https://web.expasy.org/sulfinator/ N/A Iroki https://www.iroki.net/viewer N/A IgDiscover https://gkhlab.gitlab.io/igdiscover22/ V1.0.2 ByosTM (Version 5.5) Protein Metrics Inc. https://www.proteinmetrics.com/products/ byonic/ XCalibur Version v4.2 Thermo Fisher N/A Orbitrap Fusion Tune application v3.1 Thermo Fisher N/A UCSF ChimeraX Meng et al.96 https://www.cgl.ucsf.edu/chimerax/; RRID:SCR_015872 Relion 4.0 Kimanius et al.97 RRID:SCR_016274 EPU Multigrid Thermo Fisher N/A CryoSPARC Punjani et al.98 https://cryosparc.com UCSF Chimera Pettersen et al.95 https://www.cgl.ucsf.edu/chimera/ Phenix Liebschner et al.99 https://phenix-online.org PDBePISA Krissinel and Henrick100 http://www.ebi.ac.uk/pdbe/pisa/ (Continued on next page) Immunity 59, 1140–1160.e1–e11, April 14, 2026 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Coot Emsley and Cowtan101 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ MolProbity Chen et al.102 http://molprobity.biochem.duke.edu EMRinger Barad et al.103 https://github.com/fraser-lab/EMRinger ABodyBuilder2 Abanades et al.104 https://opig.stats.ox.ac.uk/webapps/ sabdab-sabpred/sabpred/abodybuilder2/ Other 0.2 um membrane filters Fisher Scientific Cat# 564-0020 SteriflipTM Vacuum Filter Units MilliporeSigma Cat# SCGP00525 Superdex 200 Increase10/300 GL column GE Healthcare Cat# GE28-9909-44 Superose 6 Increase 10/300 GL GE Healthcare Cat# GE29-0915-96 Protein A Sepharose Cytiva Cat# 17127903 Protein G Sepharose Cytiva Cat# 17061805 TOYOPEARL AF-Tresyl-650M TOSOH Cat# 0014472 Galanthus nivalis lectin (snow drop), agarose bound Vector Labs Cat# AL-1243-5 ProA Sensor Sartorius Cat# 18-5012 SA Sensor Sartorius Cat# 18-5020 RNeasy Plus Mini Kit Qiagen Cat# 74134 Dnase 1 Qiagen Cat# 79254 Streptavidin-AF488 Thermo Fisher Cat# S32354 Streptavidin-AF647 Thermo Fisher Cat# S21374 Streptavidin-BV421 BD Biosciences Cat# 563259 FVS510 Live/Dead stain Thermo Fisher Scientific Cat# L34966 Chromium Next GEM Single Cell 5’ Kit v2 10X Genomics Cat# 1000253 Chromium Next GEM Chip K Single Cell Kit 10X Genomics Cat# 1000286 NexttSeq 1000/2000 P1 Reagent Cartridge Illumina Cat# 20072323 E-Gel 96 2% with SYVR Safe Fisher Scientificd Cat# G720802 Econo-pak column Biorad Cat# 7321010 100kDA Ultra centrifugal filter unit Millipore Cat# UFC910024 50kDA Ultra centrifugal filter unit Millipore Cat# UFC905024 10kDA Ultra centrifugal filter unit Millipore Cat# UFC901024 Pierce Fab Preparation Kit Fisher Scientific Cat# 44985 cryovials Globe Scientific Cat# 3010 Sterile vacutainers DB vacutainer Cat# 364606 cryovial Sarstedt Cat# 72.694.396 ViaStain AOPI solution Revvity Cat# CS2-0106-25ml Cell strainer Falcon Cat# 352360 5 ml round bottom tube Corning Cat# 352058 C18 ZipTip Merck Milipore Cat# ZTC18S008 Oasis HLB 96-well μElution Plate Waters Cat# 186001828BA Vivaspin 500, 3 kDa MWCO, Polyethersulfone Sigma-Aldrich Cat# GE28-9322-18 Orbitrap Fusion mass spectrometer Thermo Fisher Scientific N/A Easy nLC 1200 Thermo Fisher Scientific N/A EasySpray PepMap RSLC C18 column (75 μm x 75 cm) Thermo Fisher Scientific Cat# ES805 AcclaimTM PepMapTM 100 C18 HPLC Trap column Thermo Fisher Scientific Cat# 164946 Electron microscopy copper mesh grids Electron Microscopy Sciences Cat# EMS400-Cu Whatman #1 filter paper Cytiva Cat# 1001-320 (Continued on next page) e4 Immunity 59, 1140–1160.e1–e11, April 14, 2026 .. Expi293F cells (Gibco, Cat# A14527) were acquired from Gibco and cultured in Expi293 Expression Medium (Gibco, Cat# A1435101) at 37◦C with 8% CO2 in a 125 rpm shaker.



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    Agarose Bound Galanthus Nivalis Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    galanthus nivalis lectin conjugated agarose beads  (Vector Laboratories)
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    gnl beads  (Vector Laboratories)
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